The Agilent  HPLC-MS 6230B Time-of-Flight system delivers full-spectrum, high-resolution, accurate-mass data for screening, profiling and identifying small molecules, large biologic compounds, and everything in between.

• HPLC is equipped with a quaternary pump for reversed phase chromatography and an isocratic pump for normal phase chromatography.
• The maximum operating pressure is 600bar.
• A suite of reverse phase columns are available for use.
• The HPLC is equipped with a UV/VIS 1260 diode array detector (wavelength range 190-600nm) and a TOF-MS (an orthogonal time-of-flight analyzer).
• Two ionization sources are available for use: Dual-ESI (Electrospray) and APPI (Atmospheric Pressure Photoionization).
• Ion detection can be carried out in positive, negative and fast polarity switching modes.
• Two sample introduction modes are available: LC and direct injection.
• The software used is MassHunter Workstation version B.05.01.

The facility has five instruments to cover for a wide range of applications, from high resolution mass determination of large organic molecules, to separation, quantitation and identification of organic compounds with a number of sample introduction options ionization sources and analyzer types to allow analysis over a large mass range from small molecules to large proteins and for different compound chemistries.

The instruments currently in the facility are:

1. GC-MS (EI) with a quadrupole analyser
2. GC- MS (EI/CI) with a quadrupole analyser
3. HPLC-UV/VIS with options for normal and reverse chromatography
4. HPLC-UV/VIS-MS (TOF) with Dual ESI and APPI ionization sources
   The TOF (time of flight) analyser provides high sensitivity and resolution for accurate mass determination of small and large organic molecules. Normal or reverse phase chromatography options.
5. CE-UV/VIS with option for coupling to the MS (TOF)

Reverse phase chromatography columns are available for use in the HPLC-UV/MS applications. The full list is available on the website.

Potential users of the facility are encouraged to meet with the operator prior to training to discuss the scope and nature of their work to determine the most appropriate instrument choice.

• Biological and Life Sciences
• Biomedical
• Chemistry and Biochemistry
• Engineering - Agricultural, Forest, Environmental, Mining, Mineral
• Engineering - Chemical, Nuclear, Other
• Environmental and Earth Sciences
• Marine/Ocean Sciences
• Medical Sciences

• Biomedical
• Agriculture, Animal Science and Food
• Chemical Industries
• Clean Technology
• Consumer Non-Durables
• Education
• Energy (Renewable and Fossil)
• Environmental Technologies and Related Services
• Fisheries and Aquaculture
• Forestry and Forest-Based Industries
• Health Care and Social Services
• Life Sciences, Pharmaceuticals and Medical Equipment
• Mining, Minerals and Metals
• Ocean Industries