Equipment
The PerkinElmer LS 55 Fluorescence spectrometer offers flexibility, versatility, reliability and ease-of-use. A wide range of automated accessories have also been developed for the system making it the system ideal for bioresearchers conducting:
- Microplate-based measurements
- Polarization
- Anisotropy assays
- Intact cell work
- Analysis of protein suspensions
Principle
Computer controlled ratioing luminescence spectrometer with the capability of measuring fluorescence, phosphorescence or chemiluminescence, and bioluminescence.
Pulse rate, delay and gate times can be varied.
Wavelength accuracy
1 nm
Wavelength reproducibility spectral bandpass
+ 0.5 nm
The excitation slits 2.5-15 nm and emission slits 2.5-20 nm can be varied and selected in 0.1 nm increments.
Scanning speed
The scanning speed can be selected in increments of 1 nm from 10–1500 nm/minute.
Data can also be collected with respect to time.
Emission filters
Computer selectable cut-off filters, 290, 350, 390, 430 and 515 nm, a blank (to act as a shutter), a 1 % attenuator, and a clear beam position.
Sensitivity
Minimum signal-to-noise level using the Raman band of water, excitation 350 nm, is 750:1 RMS measuring noise on the Raman peak, and 2500:1 RMS measuring noise on the baseline.
Standard sample holder
A single position water thermostatted holder for 10 mm cuvettes.
Data analysis
Routines are available for performing mathematical calculations on stored data. These include arithmetic functions, smoothing, 1-4th order derivatives, area, peak, normalization, merge, difference, interpolate.
Specific applications programs are provided for determining intracellular ion concentration and simple quantitation.
Data system
Operating System: Windows® 7, 32 bit operating system required.
Power requirements
90 – 132 VAC, 50/60 Hz, 2A
200 – 264 VAC, 50/60 Hz, 1A
Dimensions
265 mm high, 790 mm wide, 680 mm deep (including sample compartment).
Weight 49.5 kg.
Research Facility
- Exercise Physiology Lab - Teaching and research lab
- Anatomy Lab - Teaching and research lab
- Motor Behaviour - Teaching and research lab
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Contacts
Date submitted: Fri, May 5, 2017 6:46 PM
Date updated: Fri, May 5, 2017 6:47 PM