Fluorescence Spectrometer

Equipment



Fluorescence Spectrometer
Perkin Elmer
LS55

The PerkinElmer LS 55 Fluorescence spectrometer offers flexibility, versatility, reliability and ease-of-use. A wide range of automated accessories have also been developed for the system making it the system ideal for bioresearchers conducting:

  • Microplate-based measurements
  • Polarization
  • Anisotropy assays
  • Intact cell work
  • Analysis of protein suspensions

Principle

Computer controlled ratioing luminescence spectrometer with the capability of measuring fluorescence, phosphorescence or chemiluminescence, and bioluminescence.

Pulse rate, delay and gate times can be varied.

Wavelength accuracy

1 nm

Wavelength reproducibility spectral bandpass
+ 0.5 nm

The excitation slits 2.5-15 nm and emission slits 2.5-20 nm can be varied and selected in 0.1 nm increments.

Scanning speed

The scanning speed can be selected in increments of 1 nm from 10–1500 nm/minute.

Data can also be collected with respect to time.

Emission filters

Computer selectable cut-off filters, 290, 350, 390, 430 and 515 nm, a blank (to act as a shutter), a 1 % attenuator, and a clear beam position.

Sensitivity

Minimum signal-to-noise level using the Raman band of water, excitation 350 nm, is 750:1 RMS measuring noise on the Raman peak, and 2500:1 RMS measuring noise on the baseline.

Standard sample holder

A single position water thermostatted holder for 10 mm cuvettes.

Data analysis

Routines are available for performing mathematical calculations on stored data. These include arithmetic functions, smoothing, 1-4th order derivatives, area, peak, normalization, merge, difference, interpolate.

Specific applications programs are provided for determining intracellular ion concentration and simple quantitation.

Data system

Operating System: Windows® 7, 32 bit operating system required.

Power requirements

90 – 132 VAC, 50/60 Hz, 2A

200 – 264 VAC, 50/60 Hz, 1A

Dimensions

265 mm high, 790 mm wide, 680 mm deep (including sample compartment).


Weight 49.5 kg.

2001

Research Facility



School of Kinesiology Labs
Acadia University
Wolfville
Nova Scotia (NS)
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Contacts



Ned Kelleher Primary Contact
9025851522
Lab Tech

Date submitted: Fri, May 5, 2017 6:46 PM
Date updated: Fri, May 5, 2017 6:47 PM

2018-04-09T04:42:05+00:00