Pipeline for multiplexing RT-qPCR with fluorescence microscopy and nucleotide sequencing.
- Biological and Life Sciences
- Medical Sciences
- Agriculture, Animal Science and Food
- Environmental Technologies and Related Services
- Health Care and Social Services
- Life Sciences, Pharmaceuticals and Medical Equipment
For experiments involving visulization of fluorescent labled postmortum tissue sections as well living cell cultures over extended periods of time. It is equipped with low-light digital image capture; both color and grayscale. The system is completely automated and can be programmed for long-term experiments on living cells.
CellASIC microfluidics system with the Tri-Gas mixer
Stage Chamber Temp and Gas control
Zeiss Definite Focus
Ludl indexed X/Y stage
X-Cite120LED LED fluorescence source
Pecon chamber temp control
QImaging Retiga SRV
The gold standard in high throughput DNA array and next generation sequencing verification. A method of high resolution DNA sequencing (up to 180nt) based on the 'sequencing by synthesis' principle that enables rapid and accurate quantification of sequence variation. Main application in de novo sequencing, complex mutation analysis (SNP, CNV), epigenetics (CpG and CpN analysis of DNA methylation patterns) in human and animal tissue as well as resistance typing and microbial ID.
Application software: PyroMark Q24 Advanced SW
Throughput: 1–24 samples
PCR requirements: ~0.5–3 pmol of product
Maximum read length: 10–140* bp or more depending on assay
Sensitivity: 2% mutation 98% wild-type
Date submitted: Thu, Aug 2, 2018 2:37 PM
Date updated: Thu, Aug 2, 2018 2:38 PM