The cycling of the three key chemical controls on life in the ocean (carbon, oxygen, & nitrogen) is undergoing unprecedented change in the oceans as a result of human activities. We aim to observe, understand, and predict complex interactions within these biogeochemical cycles, which will help us understand the implications of these changes for marine life and the climate.
The CERC.OCEAN laboratory is focused on chemical oceanography and in the measurement of greenhouse gases, such as nitrous oxide, carbon dioxide and halocarbons. The laboratory is equipped with high-tech instruments, such as isotope ratio mass spectrometers, gas chromatographs, cavity ring down spectrometers and nutrient autoanalyzer.
- Chemistry and Biochemistry
- Marine/Ocean Sciences
- Energy (Renewable and Fossil)
- Environmental Technologies and Related Services
- Fisheries and Aquaculture
- Ocean Industries
d15N and d13C in bulk organic and inorganic of solid samples
Filtered seawater samples or sediment samples are packed in tin capsules to obtain 80 ug N. The Vario Micro Cube is configured to run in CN mode and calibrated to obtain quantitative %C and %N. The technique consists of flash combusting samples at 1150˚C, reducing nitrogen and carbon compounds to N2 and CO2 products and separating the different elements with a temperature programmed desorption trap. These different gaseous components are then carried by ultrapure helium to the Isoprime 100 continuous flow isotope ratio mass spectrometer for d15N and d13C analysis.
Agilent 7890B Gas Chromatograph - Mass Spectrometer 5977A - Halocarbon gases concentrations
This automated, continuous flow, analysis measures Nitrite, Nitrate, Phosphate, Silicate, and Ammonia. Chemical reagents and samples, segmented with air bubbles, are pumped through a manifold to undergo treatment such as mixing, heating, etc. before entering a flow cell to be detected with either colorimetric detection (with optical background correction) or a fluorometer (Ammonia).
Freshwater and seawater samples are analyzed on this Picarro water isotope analyzer. One ul of water is injected, vaporized and introduced into the analyzer where readings of d18O and d2D are obtained using the cavity ring down spectrometry (CRDS) technique.
Four ml of sample is transferred to a cooled chamber where phosphoric acid is added for transformation of dissolved inorganic carbon to CO2 gas, which is then sparged and sent to the Picarro analyzer for analysis of carbon isotopic composition (d13C) as well as quantitative determination of dissolved inorganic carbon (DIC (uM.l)).
Measurements of nitrous oxide in air.
Date submitted: Thu, Aug 30, 2018 12:21 PM
Date updated: Thu, Aug 30, 2018 12:22 PM