Flow Cytometry CORE lab

Research Facility



Flow Cytometry CORE lab
Dalhousie University
Halifax
Nova Scotia (NS)

Our facility operates several Flow Cytometers, currently three analyzers and two sorters. At a basic level flow cytometers allow you to tag surface, or internal markers of cells with Fluorochrome labels that are exited by a range of possible lasers emitting signals over a range of particular wavelengths. Data is processed such that expression of panels of markers can be quantified in a relative manner on a single cell basis at hundreds to thousands of cells per second.

Clients of our core use flow cytometry to phenotype and quantify populations of cells in their particular biological field of study, most often immunological and pathological processes. With our two sorting Flow Cytometers it is also possible to physically separate and collect identified populations of cells for use in further characterization or use in downstream experiments. 

  • Biological and Life Sciences
  • Biomedical
  • Biotechnology
  • Chemistry and Biochemistry
  • Marine/Ocean Sciences
  • Veterinary Sciences
  • Biomedical
  • Biotechnology
  • Agriculture, Animal Science and Food
  • Chemical Industries
  • Health Care and Social Services

Contacts



Derek Rowter
cellsort@dal.ca
9024947858
Flow Cytometry Lab Manager
Carla Ross
9024941887
Director Medical Research Development Office

Equipment



Flow Cytometer
FACSCalibur

High speed analysis of immunofluorescently labeled cells

2 laser 4 colour analyzer, 488 blue and 635 nm red lasers


Flow Cytometer
FACSCanto II

High speed analysis of immunofluorescently labeled cells

3 laser 8 colour cytometer with blue 488nm, violet 405 nm, and Red 633nm lasers


Flow Cytometer
LSR Fortessa SORP

High speed analysis of immunofluorescently labeled cells

4 laser 18 colour cytometer with Blue 488nm, violet 405nm, green 532nm, and red 628nm lasers


Flow Cytometer (sorter)
FACSAria I

High speed analysis, and sorting of immunofluorescently labeled cells

2 laser 7 colour Flow Cytometer with Blue 488nm, and red 633nm lasers


Flow Cytometer (sorter)
FACSAria III

High speed analysis, and sorting of immunofluorescently labeled cells

2 laser 8 colour Flow Cytometer with Blue 488nm, and red 633nm lasers. Housed in biosafety cabinet for use with RG2 classified agents.

Date submitted: Wed, Jan 18, 2017 11:11 AM
Date updated: Thu, Jan 19, 2017 12:07 PM

2018-04-09T04:31:06+00:00