Cold-ocean Deep-sea Research Facility

Research Facility



Cold-ocean Deep-sea Research Facility
Memorial University
St. John's
Newfoundland and Labrador (NL)

The Cold-Ocean Deep-Sea Research Facility (CDRF) at the Ocean Sciences Centre provides researchers with access to state of the art infrastructure and equipment for the study of aquatic organisms, especially those from the cold waters of the Arctic and North Atlantic oceans.

The CDRF offers researchers unique facilities, including a local seawater line for a continuous flow-through water supply to a AQC3 certified biocontainment facility. The facility is newly equipped with histology, microscopy, deep-sea and disease challenge equipment.
  • Biological and Life Sciences
  • Biomedical
  • Biotechnology
  • Engineering - Industrial, Mechanical, Electrical
  • Environmental and Earth Sciences
  • Marine/Ocean Sciences
  • Medical Sciences
  • Biomedical
  • Biotechnology
  • Agriculture, Animal Science and Food
  • Education
  • Environmental Technologies and Related Services
  • Fisheries and Aquaculture
  • Life Sciences, Pharmaceuticals and Medical Equipment
  • Ocean Industries

Contacts



Stephen Hill
sjhill@mun.ca
7098643258
Manager, CDRF

Equipment



Scanning Electron Microscope
PhenomWorld
Phenom Pro

The CDRF has a PHENOM Pro Desktop Scanning Electron Microscope available to its users. This is a very easy to use SEM well suited for rapid analysis of physical and biological specimens. A cryo-stage is available to freeze wet samples. Additional tilt and rotate as well as low charge stages are also available. Integrated EDS for elemental analysis.

  • Magnification range 20-100000x
  • ​17 nm resolution
  • Full colour navigation camera 20-120x magnification,
  • Pro Suite software which includes:
  • Automated fibre measurement
  • 3D image construction
  • Automated image collection and mapping
  • Temperature controlled sample holder (-25 °C to +50 °C)
  • Motorized tilt rotation sample holder
  • Integrated elemental identification (EDS) detector
2014

Fluorescence Activated Cell Sorter
BD
Special Order (Aria platform)

Our florescence activated cell sorter (FACS) is a BD Special Order based on the Aria platform. It has a very versatile set-up and is capable of 4-way sorting of cells, viruses and other small organisms based on scatter and fluorophore detection gates.

  • 8 Colour cell sorter (configuration details)​
  • Temperature controlled sort collection chamber (4 - 42°C) that can accommodate Eppendorf tubes to 384 well plates
  • Blue (488nm), Red (633nm) and Violet (375nm) lasers
  • Four nozzle sizes (70, 85, 100 and 130 um)
2014

Deep-sea pressure vessels
Autoclave France
IPOCAMP

We have two IPOCAMP vessels which may be used to study deep-sea life at pressures up to 300 bars (equivalent to 3000 m depth). The system is housed in the deep-sea holding room, but is also ship mountable.

  • Flow through from filtered seawater lines
  • ​Chambers are 19 L; 20 cm diameter, 60 cm height
  • Temperatures from 2 to 100 °C
  • 12 L / hour flow
  • Endoscope with camera, monitor and recorder as well as fibre optic light guides
2014

Aquatic containment facility

CFIA certified AQC3 facility containing dry and wet lab space for study of aquatic pathogens and invasive species. Disease challenge experiment on marine or freshwater species with flow-through water systems, heated or ambient cold-water. Small recirculation systems are also available.

Currently configured wet space is of 12 x 500 L tanks. Smaller aquariums may be set-up in configurable space. All water is treated by filtration and UV. Dry lab spaces contain fish cell culture incubators, -80 freezers, BSCs and associated laboratory equipment.

2014

Date submitted: Fri, Jun 23, 2017 9:56 AM
Date updated: Fri, Jun 23, 2017 9:57 AM

2018-04-09T04:22:10+00:00