Flow Cytometry CORE lab

Research Facility

Flow Cytometry CORE lab
Dalhousie University
Nova Scotia (NS)

Our facility operates several Flow Cytometers, currently three analyzers and two sorters. At a basic level flow cytometers allow you to tag surface, or internal markers of cells with Fluorochrome labels that are exited by a range of possible lasers emitting signals over a range of particular wavelengths. Data is processed such that expression of panels of markers can be quantified in a relative manner on a single cell basis at hundreds to thousands of cells per second.

Clients of our core use flow cytometry to phenotype and quantify populations of cells in their particular biological field of study, most often immunological and pathological processes. With our two sorting Flow Cytometers it is also possible to physically separate and collect identified populations of cells for use in further characterization or use in downstream experiments. 

  • Biological and Life Sciences
  • Biomedical
  • Biotechnology
  • Chemistry and Biochemistry
  • Marine/Ocean Sciences
  • Veterinary Sciences
  • Biomedical
  • Biotechnology
  • Agriculture, Animal Science and Food
  • Chemical Industries
  • Health Care and Social Services

Research Facility

Derek Rowter
Flow Cytometry Lab Manager
Carla Ross
Director Medical Research Development Office

Industry Liaison Officer

Margaret Palmeter

Equipment 6 piece(s)

Flow Cytometer

High speed analysis of immunofluorescently labeled cells

2 laser 4 colour analyzer, 488 blue and 635 nm red lasers

Flow Cytometer

High speed analysis of immunofluorescently labeled cells

3 laser 8 colour cytometer with blue 488nm, violet 405 nm, and Red 633nm lasers

Flow Cytometer
LSR Fortessa SORP

High speed analysis of immunofluorescently labeled cells

4 laser 18 colour cytometer with Blue 488nm, violet 405nm, green 532nm, and red 628nm lasers

Flow Cytometer (sorter)

High speed analysis, and sorting of immunofluorescently labeled cells

2 laser 7 colour Flow Cytometer with Blue 488nm, and red 633nm lasers

Flow Cytometer (sorter)

High speed analysis, and sorting of immunofluorescently labeled cells

2 laser 8 colour Flow Cytometer with Blue 488nm, and red 633nm lasers. Housed in biosafety cabinet for use with RG2 classified agents.

Single cell genomics platform

The Fluidigm C1 is a single cell genomics tool which captures single cells in 96-800 individual sites by means of microfluidics technology. Often paried with preparation by traditional Flow Cytometric sorting (in the case of mixed cell types) the C1 allows lysis, reverse transcription, and cDNA pre amplification for downstream sequencing or qPCR from single cells. The primary application is for single cell transcriptomics.


Date submitted: Wed, Jul 25, 2018 10:57 AM
Date updated: Wed, Jul 25, 2018 10:57 AM

Skip to toolbar