Boreal Ecosystem Research Facility

Research Facility



Boreal Ecosystem Research Facility
Memorial University, Grenfell Campus
Corner Brook
Newfoundland and Labrador (NL)

The Boreal Ecosystem Research Initiative (BERI) laboratories are a multidisciplinary research center located on the Grenfell Campus of Memorial University of Newfoundland (MUN). The purpose of the BERI labs is to facilitate multidisciplinary research in the areas of Boreal ecosystem, agriculture and environmental sciences relevant to the Atlantic region of Canada.  

The facility is divided into a suite of 4 laboratories (preprocess, extraction, molecular and analytical) that house a range of equipment relating to molecular, bio-chemical, imaging, organic inorganic and field (physical soil, air and water) analyses and techniques.

BERI welcomes collaborations or access to conduct agriculture, boreal ecosystem and environmental science based research. Such collaborators include those from industry, government agencies, and academic institutions both nationally and internationally that are using the facility to advance their various research programs.

  • Biological and Life Sciences
  • Biotechnology
  • Chemistry and Biochemistry
  • Engineering - Agricultural, Forest, Environmental, Mining, Mineral
  • Environmental and Earth Sciences
  • Marine/Ocean Sciences
  • Biomedical
  • Biotechnology
  • Agriculture, Animal Science and Food
  • Chemical Industries
  • Education
  • Environmental Technologies and Related Services
  • Fisheries and Aquaculture
  • Forestry and Forest-Based Industries
  • Mining, Minerals and Metals
  • Ocean Industries

Contacts



Jamie Warren
jwarren@grenfell.mun.ca
7096394899
Research Laboratory Coordinator
Tao Yuan
7096394896
Research Laboratory Coordinator

Jamie Warrent
7096394899
Research Laboratory Coordinator

Equipment



Droplet Digital PCR system
Bio-Rad
QXX200

The QX200 Droplet Digital PCR (ddPCR™) System from Bio-Rad is used for absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications. With no need for a calibration curve this system is ideal an accurate quantification of nucleic acids at low target concentrations in soil and water.

2014

Real-Time PCR Detection System
Bio-Rad
CFX96 Touch

The Bio-Rad RT-q PCR uses real-time PCR to amplify and quantify the initial abundance of target DNA with accuracy and high sensitivity over a wide dynamic range. Real-time PCR/qPCR assays are used for rapid and sensitive determination and quantification of nucleic acid in various biological samples, with diverse applications such as measuring the abundance of Ammonium-Oxidizing bacteria in soils for nitrification purposes. Quantification of gene expression can also be completed by RT-qPCR by reverse transcribing RNA into cDNA to be measured.

2014

NanoDrop fluorospectrometers
Thermo Fisher
2000 and 3300

Measure the concentration and purity (NanoDrop 2000) or selectively verify concentrations (NanoDrop 3300) of DNA, RNA, and protein in samples.

2014

Gene Pulser Xcell™ Electroporation Systems
Bio-Rad
Gene Pulser Xcell™ Electroporation Systems

The Gene Pulser Xcell™ system is used for the transfection of every cell type. Electroporation using this system can be done for a variety of cell types and allows for efficient nucleic acid delivery. This system is integral in applications such as functional genomics, biochemical characterization, protein production and mutational analysis. 

2014

Capillary Electroporesis system
Qiagen
QIAxcel advanced system

The QIAxcel Advanced System from Qiagen automates capillary electrophoresis at a high resolution and allows for the separation and analysis of DNA, RNA and proteins.

2014

Acoustic Focusing Cytometer
Applied Biosystems by Life Technologies
Attune Acoustic Focusing Cytometer

The acoustic focusing cytometer uses ultrasonic waves to position cells into a single, focused line along the central axis of a capillary prior to the laser interrogation points for live/dead cell counting, cell sorting, biomarker detection and protein engineering.

2014

Confocal Microscope
Olympus
FV10i

The oil immersion all-in-one automated microscope allows you to set, select and capture high-resolution images of your specimen. 

2014

Media preparator
Integra
Mediaclave 10

To formulate and sterilize large batches of media in one step. 

2014

MediaJet Petri dish filler
Integra
MediaJet

Fill, label and sterilize large batches of petri dishes  

2014

Supercritical Fluid Extraction System
Waters
MV-10 ASFE

Waters' Supercritical Fluid Extraction (SFE) Systems extract chemical compounds using supercritical carbon dioxide instead of an organic solvent. The supercritical fluid state occurs when a fluid is above its critical temperature (Tc) and critical pressure (Pc), when it is between the typical gas and liquid state. Manipulating the temperature and pressure of the fluid can solubilize the material of interest and selectively extract it. The sample is placed in an extraction vessel and pressurized with CO2 to dissolve the sample. Transferred to a fraction collector, the contents are depressurized and the CO2 loses its solvating power causing the desired material to precipitate. The condensed CO2 can be recycled.

2014

Continuous Segmented Flow Analyzer
Seal
AA3

The SEAL Analytical AutoAnalyzer is used for testing the chemical properties of various substances, designed specifically for environmental sample analysis. It performs automated wet chemistry analysis using a special flow technique called segmented flow analysis (SFA). This machine is easy and rapid for environmental testing of common soil and water analytes including nitrite, nitrate and ammonia.

2014

Flow Injection Analyzer
LACHAT
QuickChem 8500

Lachat’s QuikChem® 8500 Series 2 Flow Injection Analysis System features high sample throughput and simple but rapid method changeover. It maximizes productivity in determining ionic species in a variety of sample types, from sub-ppb to percent concentrations of soil and water anions like nitrate, nitrite and ammonia and cations including calcium and magnesium.

2014

PerkinElmer CHNS Elemental Analyzer
Perkin Elmer
2400 Series II

The PerkinElmer 2400 Series II CHNS Elemental Analyzer is an organic elemental analyzer that facilitates the rapid determination of carbon, hydrogen, nitrogen, sulfur and oxygen content in organic and other types of materials. This is highly useful for the analysis and quantification of various macro-nutrients in soil to be used for agriculture.


DEENA Automated Block Digester
Thomas Cain - SEAL
DEENA II

DEENA II is an automated digestion system that fully automates the dispensing of reagents into test tubes, as well as the heating and cooling steps in the digestion process. 

2014

Automated Kjeldahl digestor
Foss
8200

To use automatic distillation for Kjeldahl nitrogen/protein, TKN, volatile acid/bases, ammonia nitrogen and more. 

2015

Portable Photosynthesis System
LICOR
LI-6400XT

LI-6400XT is used to measure the precise and accurate photosynthesis rate and CO2 gas exchange in plant leaves. LI-6400XT has the ability to measure the chlorophyll fluorescence and gas exchanges simultaneously over the same leaf area with fluorometer sensor head. LI-6400XT provides laboratory quality measurements of photosynthesis rate, transpirational rate, conductance of water and intercellular CO2 concentrations in the field grown crops by ensuring better control on environmental variables.

2013

Automated Soil Gas Flux System
LICOR
LI-8100A

The LI-8100A (LI-COR) is an automated soil flux measurement system and is used to measure the soil CO2 fluxes with varying rates of emissions in different environments. Carbon dioxide flux is important for terrestrial C cycle and accurate estimates of CO2 fluxes are crucial for estimating C budgets in different environments. Automated LI-8100A chambers are designed to minimize perturbations to the surrounding environmental conditions and measurements artifacts the natural soil respiration. Both survey and long term chambers close automatically, eliminating variations caused by manual chamber placements, providing accurate and repeatable data on CO2 flux.

2013

Portable Leaf Area Meter
LICOR
LI-3000C

The LI-3000C provides fast, non-destructive leaf area measurements in field grown crops. LI-3000C utilizes an electronic method of rectangular approximation providing 1 mm2 resolution. Leaf area, leaf length, average width, and maximum width are logged by the readout console as the scanning head is drawn over a leaf.

2013

In-Situ Root Imager
CID-Bio-Science
CI-600

CI-600 (CID Bio-Science) enables researchers to capture non-destructive, high resolution, digital images to observe the development and function of a plant’s root system. Root images are further analyzed by RootSnap software (CI-690 RootSnap) to study root development, architecture and root morphology over the period of time or whole growth period. Roots are mapped in a fraction of the time using the automated image analysis. RootSnap’s many features and the Snap-to-Root function can be used to monitor root growth, death and the occurrence of disease. More than a dozen parameters are calculated, including root length, area, volume, diameter, and angle of growth.

2014

Chlorophyll Meter
Konica Minolta
SPAD-502Plus

Chlorophyll meter (SPAD-502Plus) is used to measure the chlorophyll contents in the growing plant leaves. SPAD-502 Plus provides fast and non-destructive chlorophyll measurements.

2014

ICP-MS
Thermo Scientific
i-Cap Q

For elemental analysis of various sample matrix such as  soil, water, ores, and biological samples.

Detection limit down to sub ppb level.

2014

GC-MS
Thermo Scientific
Trace 1300 with TSQ8000 Triple Quadrupole

Analysis various organic compounds including pesticides, pollutants (PAHs, PCBs), fatty acid esters, hydrocarbons, etc.

2014

Date submitted: Thu, Jun 7, 2018 2:30 PM
Date updated: Thu, Jun 7, 2018 2:54 PM

2018-06-07T14:55:14+00:00